Table 3.

Primer information table.

Name (Unigene ID)	Primer sequence (5'-3')	homologs	references
Secondary Metabolism
PAL 1 phenylalanine ammonia lyase (03772, 01487, 04157, 00435, 00996)	GAAATGGACCCGTTGCAGAAGCC GCTTCGGCTGTTTTTCTTGCGGAAAT	PAL 1 Arabidopsis At2g37040 Lactuca AAL55242	Olsen et al. 2008 [56] Rookes et al. 2003 [57] Winkel-
PAL 2a (00151)	AGCTCCACCCCTCGAGATTC GTCACCTTCTCACCGGTCAA	PAL 2 Arabidopsis At3g53260 Lactuca AAO13347	Shirley 2001 [34]
PAL 2b (04127)	ATCGCGAGTACTTCTTCGCC GTCACCTTCTCACCGGTCAA	PAL 2 Arabidopsis At3g53260 Lactuca AAO13347	La Camera et al. 2004 [32]
Defense-related
Chitinase (00271, 03889, 03038, 04202, 03133)	TGGCTCCATCGTTACTGCATCTG AGTTGTGGGATAGCTGGATAGGTC	Chitinase Helianthus AAB57694 Chitinase, class II Arabidopsis At4g01700	Kasprzewska 2003 [37] Jwa et al. 2006 [36]
Glucanase (01113, 00896, 00032)	CGACCCGGTTAACATCAAGCTCG CGTCGAAAACTCCGTCGTCTTACC	Beta-1, 3-glucanase Arabidopsis At4g14080	Doxey et al. 2007 [35]
Standards
Actin (01058)	ACCAACATGAGAACAACCGATAC TCACACTGGTGTCATGGTCGGAAT	Actin Gossypium hirsutum AAP73454
Cytochrome C oxidase (Weller et al. 2000)	CGTCGCATTCCAGATTATCCA CAACTACGGATATATAAGAGCCAAAACTG		Weller et al. 2000 [60]
Ubiqutin	ACAACATCCAGAAGGAGTCC GCAACACAGCAAGCTTAACC

The annotation of each Centaurea stoebe unigene(s) is given followed by Unigene ID numbers in parentheses (publicly accessible from the PLAN database, http://bioinfo.noble.org/plan/, project 30060). For each annotation, forward primer sequence is listed first and reverse primer sequence is listed second. The top BLAST hits (annotation, species, accession number) for each unigene are given in the column "homologs," and references describing information about the genes or gene families are given in the right column.