Table 1.
Dose-response effect of CIP on TG1 E. coli chromosomal DNA analyzed with the Micro-Halomax® kit.
| Dose (μg/ml) | Width of dispersion (μm) | Class | Range |
|---|---|---|---|
| 0 | - | ||
| 0.003 | - | 0 | 0 |
| 0.006 | - | ||
| 0.008 | - | ||
| 0.012 | 1.3 ± 0.3 | I | ≤ 2.0 |
| 0.02 | 1.6 ± 0.3 | ||
| 0.04 | 2.5 ± 0.4 | II | 2.1 – 3.7 |
| 0.08 | 3.3 ± 0.4 | ||
| 0.1 | 5.1 ± 1.0 | III | 3.8 – 5.7 |
| 0.5 | 7.8 ± 1.4 | IV | ≥ 5.8 |
| 1 | 8.8 ± 1.6 | ||
The width of the halo of dispersion of DNA fragments is presented in μm (mean ± standard deviation). The extent of DNA damage was classified according to the width of the dispersion.