Table 2.
DNA fragmentation levels obtained in strains of E. coli with different susceptibilities to CIP.
| CIP dose | |||||
|---|---|---|---|---|---|
| Strain | Mutations | MIC | MIC 1× | MIC 10× | MIC 100× |
| C-20 | - | 0.007 | 1.5 ± 0.3 | 6.7 ± 0.8 | 10.3 ± 2.5 |
| C-15 | Ser83Leu from GyrA | 0.25 | 1.7 ± 0.3 | 6.2 ± 0.7 | 8.7 ± 1.1 |
| 1273 | Ser83Leu and Asp87Tyr from GyrA | 8 | 0 | 1.8 ± 0.3 | 2.7 ± 0.4 |
| 1383 | Ser83Leu and Asp87Tyr from GyrA and Ser80Ile and Glu84Lys from ParC | 128 | 0 | 0 | 0 |
| J53 | - | 0.007 | 1.8 ± 0.8 | 9.2 ± 1.2 | 10.4 ± 2.0 |
| J53qnrA1 | Plasmid gene J53qnrA1 | 0.25 | 1.9 ± 0.4 | 9.5 ± 1.3 | 9.8 ± 0.9 |
The level of fragmentation obtained by different CIP doses is indicated by the width of the halo of dispersion of DNA fragments and is measured in μm (mean ± standard deviation). MIC is in μg/ml.