. 2009 Feb 19;28(7):937–947. doi: 10.1038/emboj.2009.37

Table 3.

Binding affinities (K_D (nM)) of GDF-5 variants to immobilized BMPR-IAec, -IBec and -IIec

	BMPR-IA^a	BMPR-IB^a	BMPR-II^b
GDF-5	16.2±6.38	1.3±0.62	65.8±4.2
GDF-5 R57L	4.2±1.51	0.7±0.16	55.9±3.8
GDF-5 R57A	2.0±0.68	0.6±0.19	72.4±8.4
GDF-5 L60P	n.b.^c	42.3±7.32	31.9±5.9
GDF-5 ΔL56+S58T+H59L	⩾1000^d	⩾1000^d	73.0±5.4
^aThe apparent binding constant K_D was derived from calculating K_D=k_off/k_on.
^bK_D values were evaluated from the dose dependency of equilibrium binding.
^cNo binding above background levels could be detected.
^dThe values for the apparent K_D represent the lower limit estimated from the highest analyte concentration used in the SPR analysis.