Abstract
Correction to: The EMBO Journal (2009) 28, 383–393. doi:10.1038/emboj.2008.281
Since the publication of this paper, the authors have noticed an error in Table I.
Table 1.
DNA sequence analysis of TLS events across BP-G and cisPt-GG in human U2OS cells, in which the expression of specific TLS DNA polymerases was knocked-down using siRNA
| siRNA | Control | REV3L | POLK | POLH | POLK+REV3L | POLK+POLH |
|---|---|---|---|---|---|---|
| Nucleotide inserted opposite BP-G | Number of isolates | |||||
| C | 127 (74.3%) | 52 (36.1%) | 76 (68%) | 65 (69.9%) | 55 (42.3%) | 83 (60.1%) |
| A | 11 (6.4%) | 5 (4.5%) | 3 (3.2%) | 2 (1.4%) | ||
| G | 6 (3.5%) | 2 (1.4%) | 2 (1.5%) | 1 (0.7%) | ||
| T | 3 (1.8%) | 2 (1.4%) | 2 (1.8%) | 1 (0.7%) | ||
| Deletion/insertion | 24 (14%) | 88 (61.1%) | 29 (25.9%) | 25 (26.9%) | 73 (56.2%) | 51 (37%) |
| Total number | 171 (100%) | 144 (100%) | 112 (100%) | 93 (100%) | 130 (100%) | 138 (100%) |
| TLS clonesa | 147 | 56 | 83 | 68 | 57 | 87 |
| Mutagenic TLS (%)b | 13.6% | 7.1% | 8.4% | 4.4% | 3.5% | 4.6% |
| |
|
P=0.2c |
P=0.2c |
P=0.042c |
P=0.037c |
P=0.03c |
| siRNA | Control | REV3L | POLK | POLH | POLH+REV3L | POLK+POLH |
| Nucleotide inserted opposite cisPt-GG |
Number of isolates |
|||||
| CC | 98 (78.4%) | 53 (53%) | 60 (87%) | 59 (70.2%) | 50 (58.1%) | 44 (77.2%) |
| AC | 15 (12%) | 9 (9%) | 2 (2.9%) | 7 (8.3%) | 4 (4.7%) | 1 (1.8%) |
| TC | 6 (4.8%) | 3 (3.6%) | ||||
| CT | 1 (0.8%) | |||||
| CA | 2 (2.3%) | |||||
| Deletion/insertion | 5 (4%) | 38 (38%) | 7 (10.1%) | 15 (17.9%) | 30 (34.9%) | 12 (21.1%) |
| Total isolates | 125 (100%) | 100 (100%) | 69 (100%) | 84 (100%) | 86 (100%) | 57 (100%) |
| TLS clonesa | 120 | 62 | 62 | 69 | 56 | 45 |
| Mutagenic TLS (%)b | 18.3% | 14.5% | 3.2% | 14.5% | 10.7% | 2.2% |
| P=0.006c | P=0.01c | |||||
| U2OS cells were transfected with the indicated siRNAs. After incubation of 72 h, the plasmid mixtures containing the gap-lesion plasmid GP-BP-G1 or GP-cisPt-GG (kanR), along with the control gapped plasmid GP20 (cmR) and the carrier plasmid were introduced into the cells. After incubation of 8 h to allow TLS, the DNA was extracted and used to transform a recA E. coli indicator strain. GP-BP-G1 or GP-cisPt-GG (kanR) descendents were extracted from kanR colonies and subjected to DNA sequence analysis. Deletions and insertions are taken as non-TLS events. | ||||||
| aThe number of clones, excluding clones having large deletions or insertions. | ||||||
| bThe percent of mutagenic TLS is the fraction of events other than insertion of C opposite BP-G, or CC opposite cisPt-GG out of the total number of TLS events. | ||||||
| cP-value is given compared with the results obtained with cells transfected with control siRNA. Analysis was performed using the χ2 test. P-values that have reached statistical significance are presented in bold type. | ||||||
The correct table is shown here.
The authors apologize for any inconvenience caused.