Table 1.
Primer | Sequence 5’→3’ |
---|---|
1 | CAATGACTTACTGGCCACCGGGGCTGTAGACGA |
2 | CAGCTCTCTGTAGCACTGGCACTGCAAAAGGTC |
3 | ATTGCGGCTGCAGTGGGCACTGGGCAGGTAAG |
4 | AGTGGAGCATGCCTGTAATTGAACTGGGAGTGGAC |
5 | CCATCGTAACGCAGGGCTGTAAGTCTGTG |
6 | GCTAGGCGTCGACGTCATACATTGATGAGTTTGG |
7 | GCGTGGCGTGAAGCTTATGTTACCCCTCCGAGAATC |
8 | CGCCGCAGCCGAACGACCGAG |
9 | GACCGATCCTTTCTCTTTGT |
10 | TCTTTTCCCTTCAAGAGTCC |
11 | GGTATGATCAGTGACTTACTGGC |
12 | CCATCATGGGCACAGAGAC |
13 | ACCCAGAAGACTGTGGATGG |
14 | GGAGACAACCTGGTCCTCAG |
The table lists the primers used for mutagenesis, cloning, genotyping, copy number estimation, and qPCR. Restriction sites used in cloning are shown in italics.