Table 2.
Transgene copy number estimates and plasma PAI-1 levels
| Transgenic Founder (gender) | Copy# using G/C* ratio | Copy# using G/T* ratio | Avg. Copy# | Founder Plasma PAI-1 (ng/mL)† | Offspring Plasma PAI-1 (ng/mL)† |
|---|---|---|---|---|---|
| 1 (M) | 6.7 | 9.8 | 8 | 218.0±237.0 | 682.8 |
| 2 (F) | 158.3 | 150.8 | 154 | 30.7±17.4 | 5.5±2.3 |
| 3 (F) | 31.4 | 25.9 | 29 | 1268.8±717.0 | N/A |
| 4 (F) | 37.1 | 35.8 | 36 | 3.1±0.1 | 8.5±4.2 |
| 5 (M) | 5.8 | 10.9 | 8 | 80.4±53.2 | 5.9±1.1 |
| 6 (F) | 20.3 | 20.9 | 21 | 284.6±328.6 | N/A |
| 7 (M) | 4.8 | 6.0 | 5 | 280.2±323.5 | 402.8±111.6 |
| 8 (M) | 122.9 | 110.2 | 117 | 14.2±8.7 | 6.0 |
| 9 (F) | 9.7 | 9.4 | 10 | 15.2±14.7 | 5.7±1.1 |
| 10 (F) | 4.9 | 1.4 | 3 | 237.8±330.0 | N/A |
| Wild-type | - | - | - | 4.3±1.8 | |
*
Indicates the two point mutations introduced by the PAI-1 K154T substitution that were used independently for copy number determination. The values in the 2 columns correlate with r2=0.996.
†
Expressed as mean ± SD, n=2 (Founder) & n=1-4 (Offspring). The 2 columns correlate with r2=0.759.