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. Author manuscript; available in PMC: 2010 Mar 27.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Feb 10;381(1):81–83. doi: 10.1016/j.bbrc.2009.02.013

Fig. 1.

Fig. 1

Effects of high levels of glucose on miR-221 expression.

(A) RT-PCR analysis shows that high levels of glucose (25 mM) increase miR-221 expression in HUVECs and that the increase in miR-221 expression induced by high glucose levels is attenuated by transfection of the antisense inhibitory miRNA oligonucleotide AMO-221. The miRNA was isolated by using the mirVana miRNA Isolation Kit, and RT-PCR was performed by using the mirVana™ RT-PCR miRNA detection assay containing the primers specific for miR-221 or 5S rRNA. 5S rRNA was used as an internal control. The PCR products were confirmed by 3% agarose gel electrophoresis.

(B) Relative expression of miR-221 was quantified by densitometry. N = 4 for each group.