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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Int J Infect Dis. 2008 Jul 10;12(6):e49–e59. doi: 10.1016/j.ijid.2008.03.033

Figure 4.

Figure 4

Affinity purification of recombinant Ov-AEP-1 expressed in Escherichia coli as assessed by Coomassie Blue stained SDS-PAGE gel. Lane 1, uninduced E. coli cell pellet; lane 2, IPTG-induced cell pellet; lane 3, supernatant from pellet solubilized in binding buffer; lane 4, supernatant from pellet solubilized in 8 M urea in binding buffer; lane 5, affinity-purified Ov-AEP-1 in 6 M urea; lane 6, equal loading of refolded purified recombinant Ov-AEP-1 in PBS buffer; lane 7, immunoblot of recombinant Ov-AEP-1 protein probed with anti-hexaHis-tag antibody.