TABLE 1.
Crystallographic data for E. coli β148–152 mutant sliding clamp.
| Beamline | SSRL |
|---|---|
| Wavelength, Å | 1.000 |
|
| |
| Resolution, Å | 28.855 - 1.767 |
|
| |
| Space group | P1 |
|
| |
| Temperature, K | 100 |
|
| |
| Detector | Mar325 CCD |
|
| |
| Unit Cell parameters | a = 40.838, b = 64.471, c = 72.111Å α = 73.83, β = 82.72, γ = 83.76° |
|
| |
| Solvent content (%) | 43.87 |
|
| |
| Unique reflections | 59,193 |
|
| |
| I/σ(I) | 23.3 (2.03)a |
|
| |
| Average redundancy | 1.8 (1.6) |
|
| |
| Data completeness (%) | 91.8 (80.8) |
|
| |
| Mosacity (%) | 2.0 |
|
| |
| Rmerge (%) | 0.035 (0.347) |
|
| |
| Refinement: | |
| R factor (%) | 21.8 |
| Rfree (%)b | 28.3 |
| Rmsd bond distance, Å | 0.019 |
| Rmsd bond angle, ° | 2.08 |
| Average B factors, Å2 | 35.3 |
|
| |
| Ramachandran Plot: | |
| Core, % | 90.3 |
| Disallowed | 0.8 |
|
| |
| No. of protein atoms | 5,648 |
|
| |
| No. of solvent atoms | 381 |
|
| |
| PDB code | 3F1V |
a
Values in parentheses refer to highest resolution shell 1.83-1.77 Å.
b
Rfree is calculated the same way as R factor for data omitted from refinement (5% of reflections for all data).