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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Crit Care Med. 2009 May;37(5):1729–1734. doi: 10.1097/CCM.0b013e31819dee81

Fig. 5. Association of JAK-1 and gp130 following Sham Operation, CLP and 2CLP.

Fig. 5

Autoradiograms of representative immunoblots following Sham Operatopn, CLP and 2CLP. Cytosolic protein extract immunoprecipitated with an antibody to either gp130 or JAK-1, and subjected to 10% SDS-PAGE. After transfer blot probed with antibody to whichever protein had not been immunoprecipitated. The above autoradiogram reflects immunoprecipitation with an antibody to JAK-1 and detection with an antibody to gp130. Results were the same when we immunoprecipitated with an antibody to gp130 and probed with an antibody to JAK-1. Data from 24, 48 and 72hrs post procedure as these were the time points at which IL-6 signaling was impaired.