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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Neurochem. 2008 Sep 18;107(3):712–721. doi: 10.1111/j.1471-4159.2008.05622.x

Figure 3. Membrane permeability to LDH following secondary injury.

Figure 3

Membrane permeability was assessed by release of LDH (140 kD), an intracellular enzyme that leaks out of damaged cells. Spinal cord segments were incubated for 3 hours in each of the treatment groups. The compressed cords were removed and the remaining segments rinsed and placed in fresh medium. After one additional hour, samples were taken from the medium bathing spinal cord and assayed for LDH using the TOX-7 kit. Results are expressed as % control values ± SD (n = 6). Secondary injury significantly increased permeability to LDH, an effect that was attenuated by hydralazine. One-way repeated measures ANOVA and Post Hoc Newman Keul's test were used for statistical analysis. *P<0.05.