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. 2009 Feb 10;58(5):1144–1155. doi: 10.2337/db08-0882

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 4. — IL-21R^−/−NOD mice display altered cytokine production profiles. A: IFN-γ and IL-17 production by CD4⁺ T-cells upon 3 h phorbol 12-myristate 13-acetate/ionomycin stimulation of freshly isolated IL-21R^+/+ and IL-21R^−/−NOD splenocytes. B: enzyme-linked immunosorbent spot analysis of IFN-γ, IL-17, IL-4, and IL-10 production upon 72 h of in vitro unpolarized anti-CD3/28 stimulation of freshly isolated IL-21R^+/+ and IL-21R^−/−NOD splenocytes.