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. 2009 Feb 10;58(5):1144–1155. doi: 10.2337/db08-0882

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 7. — IL-21Tg mice develop spontaneous type 1 diabetes on the C57Bl/6 background, the severity of which is increased in the context of NOD alleles. A: IL-21Tg mice and wild-type littermate controls. Diabetic incidence was calculated per group as two consecutive readings over 250 mg/dl and displayed as percentage of diabetic mice per group (Tg⁻, n = 24; Tg⁺, n = 25). Diabetes incidence was scored as survival curve data and is different by log-rank test (P < 0.0001). B: Weekly measurements of blood glucose were performed on IL-21Tg mice after crossing to the IL-21R knockout (C57BL/6), and diabetes incidence was calculated (all mice Tg⁺, IL-21R^+/+, n = 7; IL-21R^+/−, n = 25; IL-21R^−/−, n = 8). Paraffin-embedded pancreatic tissue from wild-type and IL-21Tg mice was sectioned, stained with an anti-insulin polyclonal antibody, and counterstained with hematoxylin. C: Total number of insulin-positive islets per visual field. D: Individual islets were scored for the presence of peri- and intra-islet infiltration and displayed as percentage of infiltrated islets per group (<10 weeks, Tg⁻, n = 3; Tg⁺, n = 8; 10–16 weeks, Tg⁻, n = 12; Tg⁺, n = 14; >16 weeks, Tg⁻, n = 7; Tg⁺, n = 12). Original magnification: ×10. E: IL-21Tg mice on the C57Bl/6 background were crossed with the NOD strain to generate a B6×NOD F1 mixed background. Weekly measurements of blood glucose were performed on IL-21Tg littermate controls, and diabetes incidence was calculated (Tg⁻, n = 19; Tg⁺, n = 16). Diabetes incidence was scored as survival curve data and is different by logrank test (P < 0.0001). Paraffin-embedded pancreatic tissue from this cross was quantitated for total number of insulin-positive islets per visual field (F) and individual islets scored for the presence of peri- and intra-islet infiltration and displayed as percentage of infiltrated islets per group (G) (2 weeks, Tg⁻, n = 11; Tg⁺, n = 12; 3 weeks, Tg⁻, n = 15; Tg⁺, n = 7).