FIG. 4.

IL-6 blunts the insulin-mediated activation of eNOS in HAECs. Representative blots and quantification of phosphorylation (Ser¹¹⁷⁷ [A] or Thr⁴⁹⁵ [B])/total eNOS in HAECs treated with or without (PBS control) IL-6 (10 ng/ml) for 2 h, followed by treatment with or without (PBS control) insulin (100 nmol/l) for 15 min. *Difference (P < 0.05) IL-6 + insulin versus insulin; **difference (P < 0.05) IL-6 + insulin versus control (data are means ± SE; n = 3–4 replicates from 3–4 different experiments).