FIG. 5.
IL-6 blunts the insulin-mediated production of NO from HAECs and decreases insulin-stimulated capillary recruitment in vivo. Representative fluorescence (A) and quantification (B) of NO production in HAECs treated with or without (PBS control) IL-6 (10 ng/ml) for 2 h, followed by treatment with or without (PBS control) insulin (100 nmol/l) for 15 min. Cells were viewed for 10 min, and fluorescence intensity was recorded at 15-s intervals. Calcium ionophore and S-nitroso-N-acetyl-penicilamine (SNAP) were used as positive controls for NO synthesis. C: 1-MX disappearance in hindlimb muscles from rats that underwent control (vehicle) and euglycemic-hyperinsulinemic clamps (insulin; 3 mU · min–1 · kg–1 ) for 120 min with and without the infusion of IL-6 (5.0 μg · h−1 · kg−1 ). *Difference (P < 0.05) IL-6 + insulin versus insulin (A) and insulin versus all other conditions (C); **difference (P < 0.05) insulin versus control (data are means ± SE; n = 3–5 replicates from three different experiments for A and B, n = 6 for C). (A high-quality digital representation of this figure is available in the online issue.)