FIG. 3.

Islet structure and β-cell function of hGPR40 transgenic mice and nontransgenic mice. A: Islet morphology of hGPR40 transgenic (47M and 23F) and nontransgenic mice at 16 weeks of age. The sections were stained with anti-insulin, -glucagon, -GLUT2, and -proinsulin antibodies, respectively. B: β-Cell area was measured as the stained area for anti-insulin antibody. C and D: Glucose-stimulated insulin secretion in isolated islets from hGPR40 transgenic and nontransgenic mice. E: Palmitate-stimulated insulin secretion in isolated islets from hGRP40 transgenic (47M) mice and nontransgenic mice. Islets were isolated at 9 weeks from hGPR40 transgenic and nontransgenic mice fed regular diet. Five islets with similar sizes from each group (four batches in each group) were used. All values are the means ± SE. **P ≤ 0.01, *P ≤ 0.05 vs. nontransgenic mice by Student's t test. NonTg, nontransgenic; Tg, transgenic. (A high-quality digital representation of this figure is available in the online issue.)