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. 2009 Feb 10;58(5):1096–1104. doi: 10.2337/db08-1477

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 3. — Effects of Wortmannin on contraction-stimulated phosphorylation of Akt^Thr308 (A), GSK3^Ser21/9 (B), AMPK^Thr172 (C), ACC^Ser79 (D), CaMKII^Thr286 (E), PAS-AS160 (F), PAS-TBC1D1 (G), and glucose transport (H). Paired isolated rat epitrochlearis muscles were incubated with or without 500 nmol/l of Wortmannin for 30 min. Muscles were then either rested (basal) or stimulated to contract for 20 min, freeze-clamped immediately for immunoprecipitation (IP) and/or immunoblotting (IB), or used for 3-MG transport measurement. Data are the means ± SE, n = 9–17 per group. Post hoc analysis: *P < 0.05 (effect of contraction); †P < 0.05 (effect of Wortmannin). □, DMSO; ■, Wortmannin. Wort, Wortmannin.