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. 2009 Feb 2;58(5):1077–1085. doi: 10.2337/db08-0997

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 7. — Effects of HB-EGF and NAC on corneal epithelial wound healing in high-glucose conditions. A: Representative epithelial wound closure in cultured porcine corneas. A corneal epithelial wound of 5-mm diameter was made and allowed to heal in MEM containing normal glucose (NG; 5 mmol/l d-glucose) and in high glucose (HG; 25 mmol/l d-glucose) alone or in the presence of HB-EGF (HG-HB; 50 ng/ml), with (HG-HB-NAC) or without NAC (HG-NAC; 25 μmol/l) and BSO (HG-HB-NAC-BSO; 250 μmol/l) for 48 h, followed by Richardson staining to show the remaining wound. B: Remaining wound area after 48 hours. Changes in the means of the remaining wound area in pixels were analyzed by ANOVA. Data are the means ± SD of at least six corneas from three independent experiments. **P < 0.01. Scale bar = 5 mm. H, high; N, normal. (A high-quality digital representation of this figure is available in the online issue.)