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. 2009 Feb 17;58(5):1058–1066. doi: 10.2337/db08-1237

FIG. 6.

FIG. 6.

Effect of GPR119 knockdown on OEA-induced GLP-1 secretion. mGLUTag cells were transfected with scrambled siRNA (20 pmol/l, control) or GPR119 siRNA (20 pmol/l) 2 days before the experiment. Cells were then incubated with medium alone (1% DMSO, negative control) or OEA (10–15 μmol/l) for 2 h. cAMP content of cells (n = 6) (A) and GLP-1 content of media and cells (n = 9) (B) were determined by RIA. A, inset: GPR119 mRNA transcript levels were determined by quantitative RT-PCR relative to 18S transcript levels. *P < 0.05; ***P < 0.001 vs. control or vs. the Δ change in control cells, as indicated by the lines. #P < 0.05 vs. OEA treatment with scrambled siRNA.