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. 2009 Feb 10;58(5):1156–1164. doi: 10.2337/db08-0800

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 2. — Phenotype of G9Cα^−/−.NOD mice and G9RAG^−/−.NOD mice. G9Cα^−/−.NOD thymocytes (A), splenocytes (B), and PLN cells (C) were stained with anti-CD4 and -CD8. D: PLN cells were stained with anti-Vβ6 and CD8. E: Gated CD8 T-cells were stained with K^d-B15-23 (bold line) and control K^d-LLO 91–99 (dotted line) tetramer, respectively. G9RAG^−/−.NOD thymocytes (F), splenocytes (G), and PLN cells (H) were stained with anti-CD4 and -CD8. I: PLN cells were stained with anti-Vβ6 and CD8. The results represent three independent experiments. The mean (SD) values for the distribution of cell populations are as follows: A: CD4 1.43 (0.42); CD8 5.1 (1.6). B: CD4 8.28 (1.35); CD8 21.14 (4.62). C: CD4 6.28 (0.57); CD8 52.18 (9.26). F: CD4 0.85 (0.44); CD8 2.93 (1.22). G: CD4 2.21 (2.16); CD8 33.42 (6.94). H: CD4 1.18 (0.66); CD8 68.7 (13.32).