FIG. 3.

Functional responses of purified CD8 T-cells from G9Cα^−/−.NOD mice. A: Cytotoxic assay of insulin-reactive CD8 T-cells directly ex vivo (○) from G9Cα^−/−.NOD mice compared with precultured cells (●) in response to increasing B15-23 peptide concentrations at an E:T ratio of 10:1. B: Intracellular IFN-γ and TNF-α production in response to increasing concentrations of insulin B15-23 peptide after a 40-h culture. C: ³H-Thymidine incorporation proliferation assay of splenocytes from G9Cα^−/−.NOD mice in response to B15-23 peptide and to influenza hemagglutinin (flu HA) control peptide. ●, Ins B15-23; ○, flu HA. D: ³H-Thymidine incorporation proliferation assay of splenocytes from G9Cα^−/−.NOD mice in response to cross-presented insulin protein or control KLH. The results were from one of four independent experiments.