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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Thromb Haemost. 2008 Jul 19;6(10):1789–1795. doi: 10.1111/j.1538-7836.2008.03088.x

Figure 3. Structural integrity of the mutant GPIb-IX complex.

Figure 3

Cell lysates from CHOαCCβIX (CC) and CHOαSSβIX (SS) cells were immunoprecipitated with FMC25 in the buffer containing either 1% digitonin (A) or 1% triton X-100 (B), and resolved in a SDS gel under reducing conditions. After transfer, the membrane was probed separately for GPIbα, or with polyclonal antibodies for GPIbβ or GPIX. Each figure is a representative of 3–4 independent experiments.