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. 2009 Apr 30;4(4):e5410. doi: 10.1371/journal.pone.0005410

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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2 µg of the purified His-tag recombinant protein (MSP3.1u,….MSP3.8u) was dot blotted on nitrocellulose strips. Antibodies affinity-purified against each unique region recombinant protein, from hyperimmune sera (anti-MSP3.1u,….anti-MSP3.8u), were tested against a panel of all unique region recombinant proteins, as shown in the figure above. The pattern of antibody reactivity shows high specificity of the affinity-purified antibodies towards the recombinant proteins against which they were affinity-purified.