Table 2. Naturally occurring antibodies against related C-terminal regions of the MSP3 family of proteins exhibit cross-reactivity.

	MSP3.1-Ct	MSP3.2-Ct	MSP3.3-Ct	MSP3.4-Ct	MSP3.7-Ct	MSP3.8-Ct	DG571-His	BSA
anti-MSP3.1-Ct	0.156 (100%)	0.009 (5.8%)	0.052 (33.3%)	0.007 (4.5%)	0.054 (34.6%)	0.036 (23.1%)	0.005 (3.2%)	0.006 (3.8%)
anti-MSP3.2-Ct	0.073 (54.5%)	0.134 (100%)	0.029 (21.6%)	0.005 (3.7%)	0.052 (38.8%)	0.05 (37.3%)	0.005 (3.7%)	0.006 (4.5%)
anti-MSP3.3-Ct	0.135 (117.4%)	0.052 (45.2%)	0.115 (100%)	0.006 (5.2%)	0.115 (100%)	0.054 (47.0%)	0.006 (5.2%)	0.006 (5.2%)
anti-MSP3.4-Ct	0.158 (216.4%)	0.032 (43.8%)	0.095 (130.1%)	0.073 (100%)	0.107 (146.6%)	0.075 (102.7%)	0.007 (9.6%)	0.007 (9.6%)
anti-MSP3.7-Ct	0.047 (32.0%)	0.006 (4.1%)	0.005 (3.4%)	0.005 (3.4%)	0.147 (100%)	0.007 (4.8%)	0.006 (4.1%)	0.006 (4.1%)
anti-MSP3.8-Ct	0.085 (72.6%)	0.027 (23.1%)	0.031 (26.5%)	0.009 (7.7%)	0.062 (53.0%)	0.117 (100%)	0.007 (6.0%)	0.006 (5.1%)

Antibodies affinity-purified against C-terminal recombinant protein from each member of MSP3-family of proteins were assessed for their cross-reactivity towards other members by ELISA. O.D.₄₅₀ values obtained for the reactivity of affinity-purified antibodies towards each recombinant protein are shown. The shaded boxes represent reactivity of the antibodies affinity-purified against their respective recombinant proteins, which was considered to be 100%. The degree of cross-reactivity towards other members of the family is expressed as fractions of 100%, shown in bold.