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. 2009 Apr 29;4(4):e5382. doi: 10.1371/journal.pone.0005382

Figure 2. The function of siRNAs targeting the complementary region of EPR-1 and Survivin.

Figure 2

(A) Sequences of siRNAs. The sense strands of siRNAs were designed against the mRNA of the Survivin. The predicted melting free energies of the 4 terminal base pairs of each siRNA are indicated. Throughout this paper, antisense siRNA strands have been presented in black and sense siRNAs strands in red. (B, D) Twenty-four hours after HEK293 cells were transfected with siRNA, the degradation of EPR-1 and Survivin was analyzed by RT-PCR and qRT-PCR using β-actin for standardization. (C) Forty-eight hours after HEK293 cells were transfected with siRNA, the expressions of EPR-1 and Survivin were analyzed by Western blotting using β-actin for standardization.