Nylon wool purification enriches T cells through retention of B cells. Cells from lymph nodes of C57Bl/6 mice were isolated and either untreated (A) or purified using nylon wool columns (B) as described in materials and methods and stained for FACS analysis of T cell, B cell, NK cell, macrophage, and dendritic cell populations. C, Lymph node cells were purified using B220 magnetic beads or nylon wool and stained for FACS analysis of CD8 cells. Bold line represents cell type specific staining, thin line represents negative control. Data shown is from one representative experiment of three.