Table 1.
Percentage of TGR-1 fibroblast nuclei containing ssDNA foci
| Treatment | % Nuclei with ssDNA foci
|
||
|---|---|---|---|
| Total | <10 Foci | >10 Foci | |
| None | 1 | <1 | <1 |
| None | 2 | 1 | 1 |
| None | 2 | 1 | 1 |
| Denaturation | 93 | 11 | 82 |
| Cycloheximide (10 μg/ml) (for 1 hr) | 2 | 1 | 1 |
| Actinomycin D (100 ng/ml) (for 1 hr) | 3 | 1 | 2 |
| Etoposide (0.5 μg/ml) (for 1 hr) | 32 | 23 | 9 |
| Etoposide (2 μg/ml) (for 1 hr) | 43 | 29 | 14 |
| Etoposide (6 μg/ml) (for 1 hr) | 57 | 33 | 24 |
| Etoposide (20 μg/ml) (for 1 hr) | 61 | 15 | 46 |
| MMC (0.5 μg/ml) (for 1 hr) | 36 | 12 | 24 |
| 3 hr after MMC | 27 | 11 | 16 |
| 6 hr after MMC | 33 | 14 | 19 |
| 15 hr after MMC | 29 | 5 | 24 |
| 24 hr after MMC | 32 | 11 | 21 |
| 48 hr after MMC | 13 | 1 | 12 |
| 1 hr after 0.5 Gy | 21 | 16 | 5 |
| 1 hr after 1 Gy | 36 | 26 | 10 |
| 1 hr after 2 Gy | 40 | 20 | 20 |
| 1 hr after 5 Gy | 45 | 25 | 20 |
| 1 hr after 10 Gy | 49 | 15 | 34 |
| 6 hr after 1 Gy | 22 | 13 | 9 |
| 6 hr after 5 Gy | 25 | 14 | 11 |
| 24 hr after 0.5 Gy | 10 | 3 | 7 |
| 24 hr after 1 Gy | 14 | 7 | 7 |
| 24 hr after 2 Gy | 15 | 6 | 9 |
| 24 hr after 5 Gy | 21 | 10 | 11 |
| 24 hr after 10 Gy | 30 | 16 | 14 |
At least 300 nuclei were analyzed for each experiment.