Table 2.
Induction of Rad51 and ssDNA foci by DNA damage
| Cell line treatment | % Cells with
|
||||
|---|---|---|---|---|---|
| No foci | ssDNA foci (only) | Rad51 foci (only) | ssDNA and Rad51 foci*
|
||
| Total | Co+ | ||||
| TGR-1 | |||||
| 24 hr after etoposide | 15 | 42 | 5 | 38 | 19 |
| PPL | |||||
| 24 hr after etoposide | 8 | 19 | 4 | 69 | 47 |
| 24 hr after 0.5 Gy | 77 | 6 | 2 | 15 | 14 |
| 24 hr after 1 Gy | 58 | 17 | 5 | 20 | 19 |
| 24 hr after 2 Gy | 53 | 13 | 6 | 28 | 27 |
| 24 hr after 4 Gy | 42 | 28 | 2 | 28 | 26 |
| 24 hr after 8 Gy | 19 | 35 | 7 | 39 | 33 |
| 24 hr after 10 Gy | 22 | 15 | 7 | 56 | 43 |
| 6 hr after 10 Gy | 34 | 26 | 5 | 35 | 22 |
| 48 hr after 10 Gy | 3 | 53 | 2 | 42 | 35 |
| KRA | |||||
| 24 hr after 10 Gy | 28 | 18 | 9 | 45 | 30 |
| XPA | |||||
| None | 62 | 19 | 4 | 15 | 9 |
At least 300 cells were analyzed for each experiment.
*
Co+ cells show >10% colocalization of Rad51 and ssDNA foci.