Table 3.
Colocalization frequencies of ssDNA foci with Rad51 and RPA
| Probe 1 | Probe 2 | PPL fibroblasts 24 hr after 10 Gy [No. (percent) of foci per nucleus stained with]
|
XPA fibroblasts without treatment [No. (percent) of foci per nucleus stained with]
|
||||
|---|---|---|---|---|---|---|---|
| 1 and 2 | 1 only | 2 only | 1 and 2 | 1 only | 2 only | ||
| Rad51 | ssDNA | 6.8 (35%) | 5.4 (27%) | 7.0 (38%) | 10.7 (44%) | 5.4 (22%) | 8.2 (34%) |
| RPA | ssDNA | 7.5 (50%) | 3.7 (25%) | 3.8 (25%) | 9.5 (53%) | 7.2 (41%) | 1.1 (6%) |
| Rad51* | CREST* | 0.7 (3%) | 10.3 (52%) | 8.7 (45%) | 1.0 (4%) | 10.4 (47%) | 10.8 (49%) |
50 cells with at least 5 foci of each type were analyzed for each double-staining (Rad51 and ssDNA, RPA and ssDNA, Rad51 and CREST) experiment.
*
Because Rad51 foci and centromeres don’t interact functionally, Rad51–CREST double-staining foci are caused by chance associations.