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. Author manuscript; available in PMC: 2009 Apr 21.
Published in final edited form as: J Invest Dermatol. 2007 Oct 18;128(4):917–925. doi: 10.1038/sj.jid.5701099

Figure 1. Acute permeability barrier disruption by tape stripping (TS) stimulates CRAMP and mBD3 expression; upregulation is blocked by occlusion.

Figure 1

Flanks of normal hairless mice (n=3 in all groups) were sequentially tape stripped until TEWL≥10-fold higher than in untreated controls. Tape-stripped (TS) sites on some animals were immediately occluded with a vapor-impermeable (Latex) membrane. (a–h) Frozen sections (8 μm) from biopsies of CRAMP and mBD3 were immunostained as described in Materials and Methods. (i and j) mRNA was isolated from epidermis and quantitated by reverse transcription–PCR, using 18S RNA as standard (see Materials and Methods). (b and f) The levels of CRAMP and mBD3 are at baseline, likely replenished via rapid secretion of preformed lamellar body contents. (i and j) As expected, there is no significant increase in mRNA levels at this time point. At 4 hours after TS, expression of CRAMP and mBD3 increases (c and g), which is blocked by occlusion (d and h). This correlates with increased mRNA levels for both CRAMP and mBD3 at the same time point (i and j). Bars=50 μm.