Figure 7.

Transfection and overexpression of CCN3 does not affect cell cycling under the conditions tested. The number of cells for a given cell-cycle phase (labeled) is plotted in rat MCs that have either been transfected with an empty vector (A, control) or the gene for CCN3 (B, transfected) in representative experiments. Flow cytometric analysis of cellular DNA was conducted after 2 days of incubation with media containing 10% serum. Both plots are similar, indicating that transfection with the CCN3 gene causes little or no change in cell cycling. This is confirmed in C, in which the percentage of total cells is plotted for each cycle from three separate experiments. The profiles for control and CCN3-transfected cells at each cell-cycle phase are not significantly different, as determined by a group t-test.