Figure 1.

Effect of FIZZ1 treatment on lung fibroblast-expressed Notch1 signaling components. Normal murine lung fibroblasts were treated with the indicated doses of rmFIZZ1 for 1 hour, or as indicated. A: Cell lysates (20 μg protein each) were analyzed for Notch1, NIC1, Jagged1, and Hes1 proteins by Western blotting. Representative blots from three independent experiments are shown. The GAPDH protein served as a loading control. B: Total cell RNA was isolated at the indicated time points and analyzed for Jagged1 mRNA by real-time qPCR. The amount of Jagged1 mRNA was calculated as 2⁻ΔΔCT equivalent to fold change over the untreated control sample (calibrator) using GAPDH as the reference. The effect of FIZZ1 treatment was statistically significant at all time points shown, except for the 1-hour time point. Mean ± SE of triplicate samples are shown.