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. 2009 May;174(5):1745–1755. doi: 10.2353/ajpath.2009.080618

Figure 2.

Figure 2

Role of Jagged1 in FIZZ1 induction of α-SMA expression. A: Fibroblasts were transfected as indicated with either Jagged1 expression plasmid or its empty vector control, pcDNA3.1, and then treated with buffer only or FIZZ1. The cells were then harvested for analysis of α-SMA protein expression by Western blotting. B: Alternatively, cells were transfected with Jagged1 or control siRNA as indicated, and then treated with FIZZ1 or buffer only. The cell extracts were then similarly analyzed for α-SMA and NIC1 protein. C: NIC1 (NIC)- or empty vector control (Control)-transfected fibroblasts were treated with rmFIZZ1 or buffer and then analyzed for Jagged1 protein expression by Western blotting. D: Cells were similarly treated as in C, except instead of transfection with the NIC1 expression plasmid, 5 μmol/L of γ-secretase inhibitor X was used to suppress the generation of NIC. Representative blots from at least three independent experiments are shown.