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. 2009 May;174(5):1786–1798. doi: 10.2353/ajpath.2009.080864

Figure 2.

Figure 2

Expression of CHOP and GRP78 in colonic tissues of CHOP-null mice and WT mice. DSS was administered to CHOP-null mice (Chop−/−) and WT mice, as described in the legend of Figure 1. Colonic tissues were removed and total RNA was extracted. Samples were subjected to real-time RT-PCR, using a specific primer set for Chop or Grp78. Values were normalized to Gapdh, expressed relative to the control sample (ie, WT mice without DSS treatment), and given as the mean ± SEM (n = 4). A: ** or ##P < 0.01; *P < 0.05; n.s., not significant. Sections of colonic tissues were prepared and subjected to immunohistochemical analysis with an antibody against CHOP (B) or GRP78 (C). B: The right panel in each column is a ×4 magnified image of the boxed area defined in the left panel. D: Total protein was extracted from colonic tissues and analyzed by immunoblotting with an antibody against CHOP, GRP78, or actin.