Figure 6.

Involvement of Caspase-11-Caspase-1-IL-1β pathway in CHOP-dependent exacerbation of DSS-induced colitis. DSS was administered to CHOP-null mice (Chop^−/−) and wild-type mice (WT), as described in the legend of Figure 1, A and B. C and D: Peritoneal macrophages were prepared from CHOP-null mice (Chop^−/−) and WT mice and incubated with 10 ng/ml of LPS for 24 hours. A and C: The amount of IL-1β was determined by enzyme-linked immunosorbent assay. B and D: The Caspase-1 activity was measured as described in Materials and Methods. Values are mean ± SEM (n = 3 to 4). **P < 0.01; *P < 0.05; n.s., not significant.