Figure 1.

Induction of passive nephrotoxic nephritis is equivalent in SPARC^+/+ and SPARC^−/− mice. Immunofluorescence staining was performed to confirm that disease induction was similar in SPARC^+/+ and SPARC^−/− mice. Deposition of sheep IgG at day 7 was used to assess the heterologous phase of disease induction and revealed similar intensity and pattern of staining in SPARC^+/+ (A) and SPARC^−/− (B) mice. Staining for mouse IgG as an indicator of the autologous phase of disease likewise revealed comparable staining in the SPARC^+/+ (D) and SPARC^−/− (E) mice. The absence of sheep and mouse IgG deposition (C and F respectively) before disease induction confirms specificity of staining.