Figure 7.

Accumulation of syn proteins in PDMP-treated syn-overexpressing cells. P123H β-syn-overexpressing cells were transfected with vector, wild-type α-syn, or A53T α-syn. Cells were then treated with or without PDMP (25 μmol/L) for 24 hours, followed by immunoblot analysis and RT-PCR. A: Immunoblot analysis of syn proteins. The soluble and insoluble fractions of cell extracts (10 μg) were analyzed by immunoblotting using anti-β-syn, anti-α-syn, and anti-actin antibodies. Recombinant α- and β-syn proteins are used as positive controls. a: The blots are representative of at least three experiments. In b, the band intensities corresponding to α- and β-syn were quantified and corrected with the intensities of actin bands. The data are shown as % r. intensity of the value obtained from P123H β-syn-overexpressing cells transfected with either vector or wild-type α-syn without PDMP treatment and are expressed as the mean value ± SD (n = 3). *P < 0.05 versus PDMP-untreated cells. B: RT-PCR analysis of β- and α-syn mRNAs. Cyclophilin mRNA was used as an internal control. Similar results were obtained in three independent experiments and there were no significant effects of PDMP treatment (not shown).