Figure 3.

Endogenous NF-κB maintains breast cancer epithelial cell contact-independent growth and DNA synthesis. Cell cycle analyses by flow cytometry and colony formation assays were conducted in three breast cancer cell lines stably transduced with a retroviral expression vector encoding IκBαSR or a control vector. The data were shown as mean ± SEM for n > three separate experiments. A and C: Western blot for IκBα in NAFA (A) MCF10A, and MCF10A-ErbB2* (C). B and D: Cell proliferation determined by MTT assay (left), cell-cycle distribution assessed by FACS (middle), and colony formation assayed by counting the number of colonies (right). *P < 0.05; **P < 0.01.