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. 2009 May;174(5):1972–1980. doi: 10.2353/ajpath.2009.080819

Figure 3.

Figure 3

Immunofluorescence analysis of circulating cells participating in angiogenesis. A: Wound images of green (GFP+ cells), red (CD31), and blue (F4/80) were merged. In the wound angiogenesis assay, vessel walls were composed of cells that stained red (CD31-positive cells from wild-type mouse), pink (CD31- and F4/80-positive cells from wild-type mouse), and white (CD31- and F4/80-positive cells from green fluorescent mouse). Infiltration by macrophages of wild-type mouse (blue) and green fluorescent mouse (sky blue) were observed. B: In the periphery of gel foam, CD31-positive (red) and CD31- and F4/80-positive cells of wild-type mouse (pink) and green fluorescent mouse (white) were found in the vessel walls. In the middle of gel foam, where only newly formed vessels were present, only CD31- and F4/80-positive cells of wild-type mouse (pink) or green fluorescent mouse (white) were found. C: In tumor lesions, vessels were composed of CD31-positive cells of wild-type mouse (red), CD31- and F4/80-positive cells of wild-type mouse (pink), and green fluorescent mouse (white).