Table 1.

Rates of rhodopsin chromophore formation from opsin mutants at different 11-cis-retinal concentrations

Mutant	t1/2 of Rhodopsin formation, min∗
	0.33 μM	3.3 μM	33 μM
WT	28.7	2.7	0.5
W265F	648.4	79.8	6.1
W265Y	346.0	69.9	5.0
W265A	ND†	453.1	92.5
A164V	293.5	38.8	2.8

^*t_1/2 values for A₅₀₀ formation were calculated from the curve-fitted data presented in Fig. 3. 

^†Could not be determined because of undetectable absorption increase.