Skip to main content
. 2009 Apr 21;106(18):7420–7425. doi: 10.1073/pnas.0903033106

Fig. 1.

Fig. 1.

Association of unliganded and ligand-activated ERα with E-cadherin promoter and effect on E-cadherin expression. (A) Schematic representation of the Luc-reporter construction, showing location of the half-site ERE at position −164/−160 in the E-cadherin promoter. (B) The ER+ T47D cells were grown in estrogen-free medium for 72 h. Then, the cells were treated with either ethanol vehicle (−E2) or E2 for 90 min. ChIP assay was performed with anti-ERα and anti-RNApolII antibodies. Input DNA was used to normalize the results. (C) Quantitative real-time PCR was used to evaluate changes in E-cadherin mRNA level in T47D cells similarly grown and treated with either ethanol vehicle (−E2) or E2 for 90 min. (D) T47D cells were grown in estrogen-free medium for 24 h, then were transiently transfected with E-cadherin promoter-Luc vector. After 48 h, cells were treated with either ethanol vehicle (−E2) or E2 for 90 min, and luciferase activity was measured and normalized by using β-gal activity.