Figure 5.

Subcellular localization of CD36. A: Fluorescence micrograph of cultured podocytes that were exposed to PA for 24 hours. Cells were fixed, permeabilized, and stained with CD36 (diluted 1:200 and incubated for 1 hour at room temperature) using an Alexa Fluor 488-conjugated secondary antibody (diluted 1:1000 and incubated for 1 hour at room temperature). Nuclei were stained with DAPI. To verify the specificity of the antibody, cells were incubated with unspecific rabbit IgG. B: Podocytes that were exposed to PA for 24 hours were biotin-labeled on the cell surface and protein extracts (TL) without and after purification (P) with NeutrAvidin gel were separated by 1-D SDS-PAGE and transferred onto nitrocellulose. Western blotting was performed using antibodies against CD36, Mn-SOD, and α-DG. The positions of molecular mass markers are indicated at the right. Original magnifications, ×40.