Skip to main content
. 2009 Apr;174(4):1241–1251. doi: 10.2353/ajpath.2009.080814

Figure 6.

Figure 6

Double immunohistochemistry and spectral imaging analyses for pPERK, GSK-3β, and pGSK-3β. A: Double immunohistochemistry for pPERK (red) and GSK-3β (brown). B: Double immunohistochemistry for pPERK (red) and pGSK-3β (brown). Immunohistochemical signals for pPERK (red) and GSK-3β (brown) (C) were spectrally unmixed in green for GSK-3β (D) and red for pPERK (E). F: Colors were merged with the addition of spectrally unmixed hematoxylin staining indicated in blue. Arrow indicates that GSK-3β in these granules does not co-localize with pPERK. Immunohistochemical signals for pPERK (red) and pGSK-3β (brown) (G) were spectrally unmixed in red for pGSK-3β (H) and green for pPERK (I). J: Colors were merged with the addition of spectrally unmixed hematoxylin staining indicated in blue. Scale bars: 50 μm (A, B); 30 μm (C–J).