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. 2009 Apr 28;4(4):e5369. doi: 10.1371/journal.pone.0005369

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Various H. pylori strains were grown to exponential phase as described in the Materials and Methods, and RNA was isolated from iron replete and iron-depleted shock conditions. RPAs were performed using sodB, pfr, and fur riboprobes and results are displayed in Panels A, B, and D, respectively. Basal levels of fur expression relative to the level of expression in 26695 are depicted in Panel C. Fold decrease in expression for sodB and pfr, fold increase for fur, and relative levels of basal fur expression are plotted as single points for each strain with squares, diamonds, triangles, and circles. Each shape represents a biologically independent set of RNA. Median fold change is represented as a bar for each strain. The dotted-dashed line represents the 2-fold significance cut-off in Panels A, B, and D. In Panel A only, the triangles represent the average of two technical repeats on that independent set of RNA.