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. 2009 Mar 3;7:10. doi: 10.1186/1741-7007-7-10

Table 1.

Primers used for polymerase chain reaction and reverse transcriptase polymerase chain reaction amplification.

Gene Primer Sequence (5' to 3')
Δ14 Δ14-ORF-s CCCAGCAAACATGGCAGACATAGAC
Δ14-ORF-as CGCGAAACTTCTACGCACCGTAACTT
Δ14-5'RACE CGCTCATGTCAATGGTCTTCCTCAGCTT
Δ14-3'RACE ATGAACAAAGGAGACGGCACTTACGAGG

Δ11 Δ11-5'RACE GCCAAGCACAGCAAGATTCAGAAGAACG
Δ11-3'RACE GATTGGAATCACTGCTGGTGCCCATAGA
Δ11-RT-s GGCATTTCAAAATACTGCGCTCTCGTGG

16S rRNA 16S-s TGAAGGGCTGCAGTATTTTG
16S-as TCGAGGTCGCAAACTCTTTT