Table 1.
Gene | Primer | Sequence (5' to 3') |
Δ14 | Δ14-ORF-s | CCCAGCAAACATGGCAGACATAGAC |
Δ14-ORF-as | CGCGAAACTTCTACGCACCGTAACTT | |
Δ14-5'RACE | CGCTCATGTCAATGGTCTTCCTCAGCTT | |
Δ14-3'RACE | ATGAACAAAGGAGACGGCACTTACGAGG | |
Δ11 | Δ11-5'RACE | GCCAAGCACAGCAAGATTCAGAAGAACG |
Δ11-3'RACE | GATTGGAATCACTGCTGGTGCCCATAGA | |
Δ11-RT-s | GGCATTTCAAAATACTGCGCTCTCGTGG | |
16S rRNA | 16S-s | TGAAGGGCTGCAGTATTTTG |
16S-as | TCGAGGTCGCAAACTCTTTT |