Table 1.
Comparison of kinetic and fermentation parameters of E. coli W3110trpD9923 derivative strains in flask cultures
| Strain |
Final biomass (gDCW/L) |
μ (h-1) |
qGlc (gGlc/gDCW·h) |
qAnt (gAnt/gDCW·h) |
YBiom/Glc (gDCW/gGlc) |
YAnt/Glc (gAnt/gGlc) |
Final anthranilate titer (g/L) |
| W3110 trpD9923 | 1.29 ± 0.03 [16 h]a |
0.26 ± 0.04 [0–12 h]b |
0.34 ± 0.01 [0–16 h] |
0.02 ± 0.00 [8–20 h] |
0.18 ± 0.01 [0–16 h] |
0.06 ± 0.01 [8–20 h] |
0.31 ± 0.01 |
| W3110trpD9923PTS- | 1.04 ± 0.05 [44 h] |
0.09 ± 0.01 [24–36 h] |
0.13 ± 0.01 [0–44 h] |
0.03 ± 0.00 [28–52 h] |
0.18 ± 0.01 [0–44 h] |
0.12 ± 0.01 [28–52 h] |
0.70 ± 0.07 |
| W3110 trpD9923PTS-/pv5Glk5GalP | 0.94 ± 0.05 [32 h] |
0.20 ± 0.01 [8–16 h] |
0.24 ± 0.03 [0–20 h] |
0.04 ± 0.00 [8–16 h] |
0.15 ± 0.02 [0–20 h] |
0.12 ± 0.00 [8–16 h] |
0.33 ± 0.01 |
| W3110 trpD9923/pJLBaroGfbr | 1.09 ± 0.01 [16 h] |
0.20 ± 0.00 [0–12 h] |
0.30 ± 0.01 [0–12 h] |
0.07 ± 0.00 [4–12 h] |
0.28 ± 0.01 [0–12 h] |
0.16 ± 0.01 [4–12 h] |
0.44 ± 0.00 |
| W3110 trpD9923/pJLBaroGfbrtktA | 0.93 ± 0.04 [20 h] |
0.24 ± 0.00 [0–12 h] |
0.37 ± 0.02 [0–16 h] |
0.07 ± 0.00 [8–20 h] |
0.17 ± 0.01 [0–16 h] |
0.20 ± 0.05 [8–20 h] |
0.75 ± 0.04 |
| W3110 trpD9923PTS-/pJLBaroGfbrtktA | 0.39 ± 0.00 [32 h] |
0.15 ± 0.01 [4–16 h] |
0.43 ± 0.04 [0–24 h] |
0.03 ± 0.00 [12–44 h] |
0.10 ± 0.01 [0–24 h] |
0.10 ± 0.02 [12–44 h] |
0.45 ± 0.02 |
| W3110 trpD9923PTS-/pv5Glk5GalP/pJLBaroGfbrtktA | 0.79 ± 0.03 [24 h] |
0.24 ± 0.00 [4–12 h] |
0.21 ± 0.00 [0–28 h] |
0.020 ± 0.00 [20–40 h] |
0.19 ± 0.00 [0–28 h] |
0.14 ± 0.03 [20–40 h] |
0.33 ± 0.02 |
a values in brackets indicate the time maximum biomass was achieved b values in brackets indicate the period considered for calculation of kinetic parameters.