Table 2.
Comparison of kinetic and fermentation parameters of E. coli W3110trpD9923 derivative strains in fed-batch fermentor cultures
| Strain |
Final biomass (gDCW/L) |
μ (h-1) |
qGlc (gGlc/gDCW·h) |
qAnt (gAnt/gDCW·h) |
YBiom/Glc (gDCW/gGlc) |
YAnt/Glc (gAnt/gGlc) |
Final anthranilate titer (g/L) |
Final acetate titer (g/L) |
| W3110 trpD9923 | 21.35 ± 3.76 [34 h]a |
0.25 ± 0.02 [2–10 h]b |
0.17 ± 0.01 [0–34 h] |
0.01 ± 0.00 [6–30 h] |
0.27 ± 0.02 [0–34 h] |
0.08 ± 0.01 [6–30 h] |
4.21 ± 0.03 | 9.65 ± 2.17 |
| W3110 trpD9923PTS- | 23.30 ± 0.56 [62 h] |
0.10 ± 0.00 [6–18 h] |
0.06 ± 0.00 [0–62 h] |
0.01 ± 0.00 [14–72 h] |
0.28 ± 0.00 [0–62 h] |
0.15 ± 0.00 [14–72 h] |
13.30 ± 0.89 | NDc |
| W3110 trpD9923PTS-/pv5Glk5GalP | 14.66 ± 1.26 [38 h] |
0.15 ± 0.00 [6–18 h] |
0.13 ± 0.02 [0–38 h] |
0.01 ± 0.00 [10–34 h] |
0.20 ± 0.03 [0–38 h] |
0.09 ± 0.00 [10–34 h] |
5.30 ± 0.07 | ND |
| W3110 trpD9923/pJLBaroGfbr | 19.47 ± 0.12 [34 h] |
0.21 ± 0.00 [2–14 h] |
0.12 ± 0.01 [0–30 h] |
0.01 ± 0.00 [2–38 h] |
0.28 ± 0.02 [0–30 h] |
0.08 ± 0.002 [2–38 h] |
6.72 ± 0.01 | 3.18 ± 0.52 |
| W3110 trpD9923/pJLBaroGfbrtktA | 18.54 ± 0.19 [34 h] |
0.18 ± 0.01 [6–18 h] |
0.14 ± 0.01 [0–34 h] |
0.02 ± 0.00 [2–34 h] |
0.24 ± 0.02 [0–34 h] |
0.20 ± 0.00 [2–34 h] |
14.00 ± 0.07 | 0.50 ± 0.1 |
| W3110 trpD9923PTS-/pJLBaroGfbrtktA | 11.12 ± 0.25 [38 h] |
0.13 ± 0.00 [10–26 h] |
0.16 ± 0.01 [0–34 h] |
0.02 ± 0.00 [6–38 h] |
0.19 ± 0.02 [0–34 h] |
0.11 ± 0.00 [6–38 h] |
7.16 ± 0.53 | ND |
| W3110 trpD9923PTS-/pv5Glk5GalP/pJLBaroGfbrtktA | 15.40 ± 0.14 [38 h] |
0.16 ± 0.00 [10–22 h] |
0.15 ± 0.01 [0–30 h] |
0.02 ± 0.01 [10–34 h] |
0.23 ± 0.02 [0–30 h] |
0.09 ± 0.01 [10–34 h] |
5.08 ± 0.06 | ND |
a values in brackets indicate the time maximum biomass was achieved b values in brackets indicate the period considered for calculation of kinetic parameters. c ND, non detectable levels.