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. 2009 Apr 2;8:19. doi: 10.1186/1475-2859-8-19

Table 3.

Escherichia coli strains and plasmids used in this work

Strain/Plasmid Characteristics Reference
Strains
PB11 JM101 [supE, thi, Δ(lac-proAB), F'] ΔptsHI, crr::kmR, glucose- [10]
W3110 trpD9923 W3110 [F-λ- INV (rrnD-rrnE) 1] tryptophan auxotroph, randomly mutagenized by treatment with ultraviolet radiation. [5]
W3110 trpD9923 PTS- As W3110 trpD9923 but ΔptsHI, crr::kmR, glucose- This work
Plasmids
pJLBaroGfbr aroGfbr expressed from the lacUV5 promoter, lacIq and tet genes, tetracycline resistance, pACYC184 replication origin. [14]
pv5Glk5GalP glk and galP genes expressed from the trc5 promoter, spectinomycin resistance. pCL1920 replication origin. [13]
pJLBaroGfbrtktA pJLBaroGfbr derivative, containing the tktA gene with its native promoter. This work