Table 2.

Effect of organic solvents on Rand protease stability

Organic solvents	Log P *	Protease activity (U/ml)
		0 min	30 min
pyridine	0.71	22.66	0.0
butanol	0.80	23	38.3
benzene	2.0	61.9	72.7
toluene	2.5	45.7	47.9
p-xylene	3.1	54.4	49.6
n-hexane	3.5	47.22	53.9
n-decane	5.6	53.4	77.4
n-dodacane	6.0	65	55.7
n-tetradecane	7.6	44.7	56.4
n-hexadecane	8.8	61.9	56
None		37	37

* Adopted from Laane et al. [22]

Note: 25% (v/v) of organic solvents were added to the cell-free supernatant and incubated at 50°C with shaking 150 rpm for 30 min.